AMPLIFIED NUCLEIC ACID HYBRIDIZATION ASSAYS FOR HBV

摘要

IMPROVED AMPLIFIED NUCLEIC ACID HYBRIDIZATION ASSAYS FOR HBV Amplified solution-phase sandwich nucleic acid hybridization assays for HBV in which (1) analyte is hybridized in solution with sets of amplifier probe oligonucleotides and capture probe oligonucleotides each having a first segment that is complementary to a consensus HBV ds region sequence based on a multiplicity of HBV subtypes and a second segment that is complementary to a unit of an oligonucleotide multimer and an oligonucleotide bound to a solid phase, respectively (2) the resulting product is reacted with the oligonucleotide bound to a solid phase (3) the resulting product is washed to remove unbound materials (4) the bound materials are reacted with the multimer (5) the bound materials are washed to remove unbound multimer (6) the bound materials are reacted with a labeled probe complementary to the oligonucleotide units of the multimer.