| 摘要 |
<p>A method of producing synthetic oligonucleotides on a small or large scale using H-phosphonate nucleoside monomers is described. The method can be used to synthesize oligonucleotides of any length, including oligodeoxyribonucleotides and oligoribonucleotides. The method results in a coupling efficiency of greater than 97 % and consumes only two to three equivalents of monomer to activator per coupling reaction. In addition, the method does not require a separate capping step and capping reagent because the activating reagent serves a self-capping function thereby preventing elongation of failed sequences. The H-phosphonate linkages of the fully synthesized oligonucleotide can be oxidized with a variety of reagents to obtain either phosphate diester or other types of modified oligonucleotides.</p> |
