| 摘要 |
PURPOSE:To improve the response speed of the sensor by coating the surface of a conductive base body with an oxygen-reduction catalyst function film and enzyme film. CONSTITUTION:The base body which is a conductive carbon material is coated with an insulating 'Teflon(R)' tube around the base body and an insulating adhesive agent is packed into the spacing to form a capillary electrode. A part of the tip thereof is cut and polished to form the electrode base body 1 of a very small carbon disk type. A Co- TAPP is dropped at 2.4mul (in CH3CN) and a 50mM phosphoric acid buffer soln. contg. GOx and BSA is dropped at 2.0mul onto two pieces of the electrodes 1 by a microsyringe 2 and are mixed by a needle tip. Further, 25wt.% glutaraldehyde is dropped at 1.2mul thereon by a microsyringe 3 and is uniformly mixed by a needle tip 4. The reaction is effected for 15 minutes in atm. The mixture is then brought into reaction in a 10mM phosphoric acid buffer soln. (pH=7.0) for 12hr and the sensor is immersed for 15 minutes into an aq. 10wt.% glycine soln. to remove the unreacted glutaraldehyde. The easy formation of the very small base body is executed in this way and the response speed is extremely speeded up. The measurement with good accuracy is possible. |
