| 摘要 |
A method of immunologically assaying a human thrombin-antithrombin III complex in a human assay sample by using a first antibody composed of an insoluble carrier and an anti-human thrombin polyclonal antibody fixed to the insoluble carrier and a second antibody composed of an anti-human antithrombin III antibody and a labelling substance bound thereto; wherein (1) the anti-human thrombin polyclonal antibody has (a) a dissociation constant with respect to human thrombin of 2.5 x 10<-><9>M to 3.5 x 10<-><9>M, (b) a dissociation constant with respect to human prothrombin of 4.5 x 10<-><7>M to 5.5 x 10<-><7>M, (c) a dissociation constant with respect to the human thrombin-antithrombin III complex of 0.5 x 10<-><9>M to 1.5 x 10<-><9>M, and (d) a cross-reactivity with human prothrombin of not more than 0.05 %, and (2) the anti-human thrombin polyclonal antibody is bound to the insoluble carrier at a rate of 2 to 25 % based on the saturated amount of the polyclonal antibody bound to the insoluble carrier, and a kit therefor. e |
