PRODUCTION OF ANTI-BRAIN DERIVED ALKALI PHOSPHATASE I SPECIFIC ANTIBODY

摘要

PURPOSE:To obtain the brain derived alkali phosphatase (ALP)I specific antibody from the antiserum obtd. by immunizing the brain derived ALPI type isozyme having specific physicochemical properties to an animal. CONSTITUTION:The brain derived ALPI type isozyme which has about 15,000 mol.wt. heat resistance (56 deg.C, 5 minutes), about 60% residual activity, does not hinder phenyl alanine, and has the physicochemical properties, such as immobility in migratability in Triton X treatment and immobility in migratability in neuraminidase treatment is dosed to the animal to immunize. The antibodies which react with the isozymes of the non-brain derived ALPI type, II type and III type in addition to the anti-brain derived ALPI specific antibody reacting specifically with the brain derived ALPI type isozyme are included in the resulted antiserum. The antibodies reacting with the non-brain derived I type, II type and III type isozymes are removed and the anti-brain derived ALPI specific antibody is obtd. if this antiserum is absorbed by using the non-brain derived ALPII type and III type isozymes.