发明名称 |
Process for recovering microbially produced interferon- beta |
摘要 |
An approved procedure for the purification and renaturation of biologically active, bacterially produced IFN- beta is described. The partially purified material obtained by solubilization of isolated refractile bodies from the recombinant cells is treated to obtain reduction of the protein in the presence of a chaotropic environment and then oxidized after removal of the reducing agent. However, the chaotropic environment is retained during the oxidation. Upon removal of the chaotropic environment, a solubilizing additive is supplied to maintain the IFN- beta in solution. Further purification by conventional means may also be effected.
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申请公布号 |
US4961969(A) |
申请公布日期 |
1990.10.09 |
申请号 |
US19870048686 |
申请日期 |
1987.05.11 |
申请人 |
CETUS CORPORATION |
发明人 |
HERSHENSON, SUSAN;SHAKED, ZE'EV;THOMSON, JODY |
分类号 |
C07K1/113;C07K14/565 |
主分类号 |
C07K1/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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