发明名称 |
METHOD OF IMMUNOLOGICALLY ASSAYING HUMAN THROMBIN-ANTITHROMBIN III COMPLEX, ASSAY REAGENT AND KIT THEREFOR |
摘要 |
A method of immunologically assaying a human thrombin-antithrombin III complex in a human assay sample by using a first antibody composed of an insoluble carrier and an anti-human thrombin polyclonal antibody fixed to the insoluble carrier and a second antibody composed of an anti-human antithrombin III antibody and a labelling substance bound thereto; wherein (1) the anti-human thrombin polyclonal antibody has (a) a dissociation constant with respect to human thrombin of 2.5 x 10-9M to 3.5 x 10-9M, (b) a dissociation constant with respect to human prothrombin of 4.5 x 10-7M to 5.5 x 10-7M, (c) a dissociation constant with respect to the human thrombin-antithrombin III complex of 0.5 x 10-9M to 1.5 x 10-9M, and (d) a cross-reactivity with human prothrombin of not more than 0.05 %, and (2) the anti-human thrombin polyclonal antibody is bound to the insoluble carrier at a rate of 2 to 25 % based on the saturated amount of the polyclonal antibody bound to the insoluble carrier, and a kit therefor.
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申请公布号 |
CA2014016(A1) |
申请公布日期 |
1990.10.07 |
申请号 |
CA19902014016 |
申请日期 |
1990.04.06 |
申请人 |
TEIJIN LIMITED |
发明人 |
KOIKE, YUKIYA;MURAKAMI, TOSHINOBU;SUMI, YOSHIHIKO;ICHIKAWA, YATARO |
分类号 |
A61K39/395;C12N9/74;G01N33/86;(IPC1-7):C12N11/18;G01N33/543;G01N33/573 |
主分类号 |
A61K39/395 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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