摘要 |
<p>PURPOSE:To efficiently produce a betacyanin-based dyestuff having stable quality by culturing a callus derived from a plant of Beta vulgaris L. forming a betacyanin-based dyestuff in a liquid medium containing a reducing agent. CONSTITUTION:A callus containing a betacyanin-based dyestuff derived from a plant of Beta vulgaris L. forming a betacyanin-based dyestuff such as red beet or table beet, representatively Detroit dark red is multiplied in Murashige- Skoog medium. The callus is cultured in a liquid medium containing a reducing agent to produce a betacyanin-based dyestuff. Glutathione, ascorbic acid, sodium sulfite, etc., may be cited as the concrete example of the above-mentioned reducing agent and the amount of the reducing agent used is usually 0.1-100ppm. preferably 0.5-50ppm calculated as concentration in the medium.</p> |