发明名称 |
CLONING AND EXPRESSION OF TRANSFORMING GROWTH FACTOR BETA-2 |
摘要 |
cDNA clones coding for TGF-.beta.2 which are used to construct expression vectors capable of directing the highlevel expression of mature, biologically active TGF-.beta.2, as well as precursor TGF-.beta.2 forms, in transfected Chinese Hamster Ovary cells (CHO cells) and transfected COS cells are described. CHO and COS transfectants secreting TGF-.beta.2 at high levels are also described. CHO cells transfected with a plasmid vector carrying the complete 414 amino acid simian TGF-.beta.2 precursor secrete approximately 5 .mu.g per ml culture media. |
申请公布号 |
ZA8909623(B) |
申请公布日期 |
1990.09.26 |
申请号 |
ZA19890009623 |
申请日期 |
1989.12.15 |
申请人 |
ONCOGEN LIMITED PARTNERSHIP |
发明人 |
ANTHONY F. PURCHIO;LINDA MADISEN;NANCY WEBB |
分类号 |
A61K38/00;A61K38/18;A61P35/00;C07K;C07K14/00;C07K14/495;C07K14/52;C12M;C12N;C12N5/00;C12N5/10;C12N15/09;C12N15/12;C12N15/18;C12N15/85;C12N15/86;C12P;C12P21/02;C12R1/91 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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