发明名称 |
Intermediates |
摘要 |
A method is provided for the high fidelity, rapid and economical in vitro synthesis of oligonucleotides. Nucleoside H-phosphonates are condensed in seriatim using a dehydrating agent to produce a poly (nucleoside H-phosphonate). The produce is oxidized to yield the desired oligonucleotide. A novel reagent is provided for multiple nucleoside additions in single cycles.
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申请公布号 |
US4959463(A) |
申请公布日期 |
1990.09.25 |
申请号 |
US19860910048 |
申请日期 |
1986.09.19 |
申请人 |
GENENTECH, INC. |
发明人 |
FROEHLER, BRIAN C.;MATTEUCCI, MARK D. |
分类号 |
C07H1/00;C07H21/00;C07H21/02;C07H21/04;C12N15/00;C12N15/09;C12Q1/68 |
主分类号 |
C07H1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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