发明名称 |
DNA CONSTRUCT AND MODIFIED YEAST |
摘要 |
A DNA construct is described which comprises DNA encoding for a beta -galactosidase which can be secreted by a yeast, said DNA being flanked at its 5' end in reading frame by a yeast promoter sequence. Such DNA constructs can be incorporated in a plasmid vector (e.g. pVK11) and inserted into a suitable host, such as a yeast (e.g. Saccharomyces cerevisiae), to produce a microorganism which synthesises and secretes beta -galactosidase extracellularly or into the periplasmic space and which can grow on a substrate containing lactose, e.g. whey or whey permeate. The DNA coding for the beta -galactosidase may be of fungal origin, for example it may be derived from Aspergillus niger in the form of its lacA+ gene. |
申请公布号 |
WO9010703(A1) |
申请公布日期 |
1990.09.20 |
申请号 |
WO1990GB00373 |
申请日期 |
1990.03.13 |
申请人 |
IMPERIAL COLLEGE OF SCIENCE, TECHNOLOGY AND MEDICI |
发明人 |
HARTLEY, BRIAN, SELBY;RAMAKRISHNAN, SUNDARAM;KUMAR, VIJAY |
分类号 |
A23K3/03;C12N1/19;C12N9/38;C12N15/56;C12N15/81;C12P7/06 |
主分类号 |
A23K3/03 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|