Methods for purification, amplification and detection of a nucleic acid.
摘要
<p>A targeted nucleic acid in a biological specimen can be purified and prepared for amplification or other uses by contacting the specimen with a purification reagent specific for that nucleic acid. This reagent comprises a nucleic acid fragment complementary to the targeted nucleic acid, which fragment is attached to nonporous, nonmagnetic particles. An insoluble hybrid is thus formed which is then separated from the specimen. After dehybridization, the purified targeted nucleic acid is available for further use, such as amplification by polymerase chain reaction and detection.</p>
申请公布号
EP0388171(A2)
申请公布日期
1990.09.19
申请号
EP19900302704
申请日期
1990.03.14
申请人
EASTMAN KODAK COMPANY;CETUS CORPORATION
发明人
OAKES, FRED TERRY C/O EASTMAN KODAK CO., PAT.DEPT.;BURDICK, BRENT ARTHUR, C/O EASTMAN KODAK CO.P.DEPT;FYLES, JANET LINN, C/O EASTMAN KODAK CO., P.DEPT.;CHANG, CHU-AN, C/O EASTMAN KODAK CO., PAT.DEPT.,