发明名称 |
Replicative RNA reporter systems |
摘要 |
Highly sensitive methods for assaying for biopolymers, such as by immunoassay or nucleic acid probe hybridization assay, and compositions for carrying out the methods, are provided. The methods employ as reporter group a RNA capable of being autocatalytically replicated by an RNA-dependent RNA polymerase, such as the replicase of bacteriophage Q beta . The high sensitivity of the assay methods is due to the rapid, exponential increase in concentration of such a replicative RNA, associated specifically with biopolymer analyte, that can be achieved by autocatalytic RNA replication.
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申请公布号 |
US4957858(A) |
申请公布日期 |
1990.09.18 |
申请号 |
US19860852692 |
申请日期 |
1986.04.16 |
申请人 |
THE SALK INSTUTE FOR BIOLOGICAL STUDIES;THE TRUSTEES OF COLUMBIA UNIVERSITY |
发明人 |
CHU, BARBARA;KRAMER, FRED R.;LIZARDI, PAUL;ORGEL, LESLIE E. |
分类号 |
C12N15/09;C07H21/00;C07H21/02;C12Q1/68;G01N33/532 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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地址 |
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