发明名称 |
CELL CULTURE MEDIUM AND ITS USE IN CULTURING MAMMALIAN CELLS |
摘要 |
<p>The present invention relates to cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effective cell growth promoting amount of glucose; an effective amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyronine to increase DNA synthesis in cells; and an effective growth promoting amount of mammalian hormones and mitogenic factors, including lipoprotein, cholesterol, phospholipids and fatty acids.</p> |
申请公布号 |
IL92703(D0) |
申请公布日期 |
1990.09.17 |
申请号 |
IL19890092703 |
申请日期 |
1989.12.14 |
申请人 |
THE UNITED STATES OF AMERICA, DEPARTMENT OF COMMERCE |
发明人 |
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分类号 |
C12N5/071;C12Q1/04;G01N33/50;(IPC1-7):C12N/ |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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