| 摘要 |
PURPOSE:To obtain the title enzyme giving high solvent resistance, functioning as a catalyst of high efficiency for peptide synthesis in particular by introducing a specific group into the amino group in an enzyme. CONSTITUTION:The objective enzyme can be obtained by introducing a R-CO- group into an enzyme at such an amount as to be 10-65wt.% based on the total amino group in the enzyme (where, R is benzyloxy, t-butoxy, 9- fluorenylmethoxy, p-methoxybenzyloxy or 1-17C alkyl). The chemical modification is preferably made in an aqueous solution of pH 4.0-12.0 using a sulfonium salt of formula (R is the same as described above) (the reaction will proceed even at temperatures below room temperature). The original enzyme is pref. a protease such as chymotrypsin, trypsin, thermolysine or pepsin. |
