| 摘要 |
<p>PURPOSE:To obtain a new D-aminoacylase specifically acting on N-acetyl-D- glutamic acid by culturing a bacterium belonging to the genus Alkaligenes, capable of producing D-aminoacylase in a medium containing the glutamic acid derivative. CONSTITUTION:The above-mentioned bacterium capable of producing D- aminoacylase is cultured in a medium containing N-acetyl-D-glutamic acid and using preferably peptone as a carbon and a nitrogen sources. The concentration of the glutamic acid derivative in the medium in the culture is preferably about 0.2%, the medium has preferably pH about 7 and D-aminoacylase can be produced in the cells by culture about one day. In the culture, a culture mixture is centrifuged to collect the cells, a cell-free extracted solution is obtained by ultrasonic grinding and further D-aminoacylase is isolated and purified by a well-known method. The D-aminoacylase acts on N-acetyl-D- glutamic acid to form glutamic acid and acetic acid and has physical and chemical properties of pH 6.8-7.2, 45-50 deg.C optimum temperature of activity manifestation, etc.</p> |
