| 摘要 |
A method is provided for amplifying, mapping and sequencing nucleic acid which employs an origin linker comprising a bacteriophage origin of replication conjugated to a predetermined oligonucleotide sequence. In a preferred embodiment of the invention, nucleic acid to be mapped is digested with a restriction enzyme that leaves single stranded terminii of random sequence, after which the nucleic acid is amplified by the use of a plurality of such origin-linkers in which the linker is cohesive with the random terminii, thereby amplifying specific fragments of the nucleic acid to be mapped. The relative positions of the amplified fragments are determined by sequencing and mapping. |
