发明名称 |
YEAST PROCESSING SYSTEM COMPRISING A NEGATIVELY CHARGED AMINO ACID ADJACENT TO THE PROCESSING SITE |
摘要 |
A polypeptide for production in yeast comprises a fusion of a signal peptide, a leader peptide and a heterologous protein or polypeptide. The polypeptide is modified in its amino acid sequence adjacent to a yeast processing site positioned between the C-terminal end of the leader peptide and the N-terminal end of the heterologous protein so as to provide a presentation of the processing site which makes it accessible to proteolytic cleavage. Such a presentation is provided by adding one or more amino acids (at least one of which is negatively charged) to either the C-terminal end of the leader or the N-terminal end of the protein, or both. The heterologous protein may, for instance, be aprotinin or insulin precursor or an analogue thereof. |
申请公布号 |
WO9010075(A1) |
申请公布日期 |
1990.09.07 |
申请号 |
WO1990DK00058 |
申请日期 |
1990.03.01 |
申请人 |
NOVO-NORDISK A/S |
发明人 |
BJOERN, SOEREN;NORRIS, KJELD;NORRIS, FANNY |
分类号 |
C12N15/81;C07H21/04;C07K;C07K1/00;C07K14/00;C07K14/62;C07K14/81;C07K19/00;C12N1/16;C12N1/19;C12N15/00;C12N15/09;C12N15/31;C12N15/62;C12P21/02;C12R1/865 |
主分类号 |
C12N15/81 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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