RECOMBINANT PLASMID DNA pAA 1213-23 AND pAC 262-33 CODING FOR SYNTHESIS OF HUMAN INTERLEUKIN-2, METHOD OF THEIR CONSTRUCTION AND STRAIN OF BACTERIA ESCHERICHIA COLI RRI/pAC 262-33 AS PRODUCER OF HUMAN INTERLEUKIN-2

摘要

A recombinant plasmid DNA pAA 1213-23 coding for synthesis of human interleukin-2 has a size of 5,150 b.p. and consists of the following elements: an EcoRI-SalI fragment, of a size of 3,710 b.p. of vector plasmid pBR 322; a Cfr 13 fragment of a size of 880 b.p. containing a promoter of tryptophan operon, a polylinker section of recognition of restrictase EcoRI, SmaI, BamHI, initiating ATG and first GCC codons of mature interleukin-2; a Cfr 13-SalI fragment of a size of 570 b.p. of cloned cDNA. A recombinant plasmid DNA pAC 262-33 on the basis of said DNA pAA 1213-23 has a size of 5,135 b.p. and consists of the following elements: an EcoRI-SalI fragment of a size of 3,710 b.p. of vector plasmid pBR 322; a Cfr 13 fragment of a size of 867 b.p.; a Cfr 13-SalI fragment of a size of 570 b.p. of cloned cDNA. A method of construction of recombinant plasmid DNA pAC 262-33 comprises the preliminary construction of plasmid DNA pAA 1213-23 with subsequent construction, on its basis, of plasmid pAC 262-33 with a changed distance between the Shine-Dalgarno sequence and the initiating ATG codon. A strain Escherichia coli RRI/pAC 262-33 as producer of interleukin-2 deposited on 10.07.87 under No VKPM B-3823 at the USSR-Union Collection of Industrial Microorganisms of the USSR Research Institute for Genetics and Industrial Microorganisms Breeding.