A novel exo-type hydrolase and a method of manufacturing inulotriose and/or inulotetrose.

摘要

The present invention is directed to an exo-type hydrolase capable of hydrolyzing a fructan every 3 or 4 sugar units from the terminal fructose thereof to produce inulotriose or inulotetrose respectively; to a method of manufacturing the exo-type hydrolase, comprising the steps of culturing a microorganism which belongs the genus Streptomyces or Aureobacterium and produces the hydrolase, and of recovering the hydrolase produced; to a method of manufacturing inulotriose or inulotetrose, comprising the steps of hydrolyzing a fructan specifically with the exo-type hydrolase to produce inulotriose or inulotetrose, and of recovering the inulotriose or inulotetrose; and to streptomyces sp. MCI 2423 (FERM BP-2678) and Aureobacterium sp. MCI 2494 (FERM BP-2679).