| 摘要 |
<p>PURPOSE:To enable diagnosis of human papilloma virus(HPV) infectious diseases by culturing and proliferating a hybridoma prepared by fusing a specific splenic cell to a mouse myelomatous cell. CONSTITUTION:An HPV1 type is treated with an alkali at pH10-11 to provide an antigen, which is then administered to immunize a BALB/c mouse. The spleen thereof is then subjected to extirpation treatment to afford an antibody- producing splenic cell (A), which is subsequently mixed with a myelomatous cell (B) derived from a mouse at (1-20):1 ratio and cultured in a microplate well to provide a hybridoma group. The resultant group is then cloned to afford a hybridoma (C) capable of producing an antihuman papilloma virus monoclonal antibody (KI H8). The component (C) is subsequently cultured in a culture medium containing bovine fetal blood serum at about 37 deg.C in a CO2-containing air atmosphere for 10-14 days to provide a culture supernatant, which is then purified to produce the above-mentioned monoclonal antibody. The component (C), as necessary, is proliferated in a mouse abdominal cavity and an antibody is subsequently separated and recovered from the resultant mouse ascitic fluid.</p> |
