A METHOD FOR PRODUCING PERTUSSIS TOXIN SUBUNITS

摘要

A method for the production of a pertussis toxin subunit and a recombinant DNA molecule for the same capable of expression in a Bacillus host comprising: a part of the regulation and secretory protein signal sequence of a gene encoding a secretory protein of a Bacillus strain, operatively linked to a DNA sequence encoding a pertussis toxin subunit; DNA vectors containing said recombinant DNA molecule, bacillary hosts transformed with said DNA vector, a method for producing a recombinant DNA molecule in a Bacillus host employing said DNA vector and a polypeptide and composition of the same. By means of the invention several milligrams of the product are produced per liter of culture.