| 摘要 |
<p>PURPOSE:To efficiently produce a monoclonal antibody effective to ganglioside by synthesiziig hybridoma capable of producing a monoclonal antibody specifically recognizing specific two gangliosides. CONSTITUTION:A ceramide part of ganglioside antigen is embedded in a liposome membrane to enhance antigenicity. Further, the antigen is immunized to mouse having possibility sensitized by natural antigen to provide a monoclonal antibody (SK-8) having high potency. The monoclonal antibody can recognize both of 1V<3>(NeuAc)nLc4Cer and 1V<3>(NeuAc)Lc4Cer and has specificity unreactive with sugar lipid analogous to other gangliosides. Therefore, the antibody is extremely useful in ultramicroanalysis of neolactotetraocyl based ganglioside antigen, etc.</p> |
