Microwave method for preparation of serum samples for b-12 and folate assays.

摘要

The present invention provides novel processes for the release of vitamin B12 and/or folate in mammalian serum samples from endogenous serum binders comprising microwaving the serum samples prior to assaying the serum samples for vitamin B12 and/or folate. Prior to the assay procedure, the serum samples are typically microwaved for about 20-90 seconds, most preferably for about 60 seconds at a high setting or such that the temperature of the serum samples is brought to a temperature of about 65 to 100 DEG C, most preferably 80-85 DEG C, for at least 15 seconds.