Highly sensitive immunological detection method - esp. for low mol.wt. pollutants, with extra wash stage between addn. of sample and tracer

摘要

In an immunological detection method, the new feature is that between addn. of sample antigen and tracer, additional washing steps are carried out. Pref. the method can be applied to systems where a radioisotope, enzyme, fluorescent substance or an electronic-signal generating cpd. is used as label. USE/ADVANTAGE - The method is used to detect low mol.wt. cpds., esp. environmentally important cpds. such as plant protection agents in drinking water. The extra washing step eliminates both cooperative and competitive effects in the test, so that reagent requirements are relaxed, esp. unpurified polyclonal antibodies can be used and the degree of antigen substitution on the enzyme is less critical. The detection sensitivity is icnreased, e.g. a limit of 1ng/l in an enzyme-immunoassay system using a single photo-optical measurement. The test kits are simpler and less expensive to produce.