METHOD OF FREEZING RED BLOOD CELLS

摘要

2046627 9009184 PCTABS00001 A method of freezing a standard donor unit of red blood cells such that it can be stored for long periods and subsequently recovered in a form pure enough for transfusion purposes involves centrifuging a blood unit to remove plasma and platelets and to provide a Packed Cell Volume of the red blood cells of not less than 90 %, adding the red blood cells to a freezing bag (13), containing HES solution such that the ratio of HES/red blood cells is no more than 7 % (preferably 6 %) w/v, positioning the freezing bag (13) in a freezing frame (20, 21) adapted to maintain the thickness of the contents of the bag constant, and placing the frame (20, 21) without shaking, into liquid nitrogen.