A METHOD FOR THE DETERMINATION OF BILIRUBIN IN SOLUTION

摘要

A novel analytical method for bilirubin in biological fluids. Bilirubin is catalytically oxidized to biliverdin in the presence of bilirubin oxidase (BOX) and the latter is revertedly back-converted to initial bilirubin in the presence of suitable reducing agents. The corresponding oxidation of said reducing agents provides a signal which is monitored and provides the desired analytical data. When using selected organic fluoro-compound, said signal is provided by the release of F<-> ions which can be measured electrometrically.