发明名称 |
DETECTION OF ANTIBODIES IN BODY FLUIDS FOR DIAGNOSTIC TESTING |
摘要 |
2045673 9008957 PCTABS00001 A method for detection for screening and diagnostic purposes of antibodies in a body fluid by means of an antibody-binding protein covalently bound to a porous matrix trapped within a transparent column. A test fluid containing antibody is contacted with the protein-bound matrix whereby the protein reacts with to immobilize any antibody present in the test fluid. A biotinylated antigen of interest is contacted with the immobilized antibody whereby the biotinylated antigen binds to the immobilized antibody. Avidin covalently linked to an enzyme is contacted with the immobilized biotinylated antigen-immobilized antibody-protein bound matrix complex whereby the enzyme-labelled avidin binds to the immobilized complex. This immobilized complex is contacted with a substrate solution wherein the enzyme linked to avidin catalyzes a reaction of the substrate and produces a detectable reaction product which correlates to the presence of the antibody to be detected.
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申请公布号 |
CA2045673(A1) |
申请公布日期 |
1990.07.31 |
申请号 |
CA19902045673 |
申请日期 |
1990.01.25 |
申请人 |
EPITOPE, INC. |
发明人 |
THIEME, THOMAS R.;FERRO, ADOLPH;FELLMAN, JACK H.;GAVOJDEA, STEFAN |
分类号 |
G01N33/53;A61K47/48;G01N;G01N33/543;G01N33/547;G01N33/548;G01N33/566;G01N33/58;(IPC1-7):G01N33/543 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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