发明名称 |
Yeast promoter and process for preparing heterologous protein |
摘要 |
A method of producing a heterologous protein in yeast is disclosed, which comprises transforming a yeast Saccharomyces cerevisiae with recombinant DNA comprising a promoter selected from the group consisting of a yeast promoter or a hybrid promoter derived from a yeast promoter, particularly a hybrid promoter containing the enhancer region of SV40 virus, and a gene coding for a heterologous protein such as HBsAg and Pre S-HBsAg, particularly full length Pre S-HBsAg culturing, the resulting transformed cells to express the gene coding the heterologous protein, and isolating the heterologous protein from the cultured medium. GAP-DH and PH05 promoters and miniaturized ones can be used as the yeast promoter. The expression of the full length Pre S-HBsAg is carried out in the same expression system as that for 2nd or 3rd Pre S-ABsAg.
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申请公布号 |
US4945046(A) |
申请公布日期 |
1990.07.31 |
申请号 |
US19870057143 |
申请日期 |
1987.06.03 |
申请人 |
THE GREEN CROSS CORPORATION |
发明人 |
HORII, HAJIME;KAWABE, HARUHIDE;ARIMURA, HIROFUMI;MUKAI, HIROMICHI;KOBAYASHI, KAORU;TSUJIKAWA, MUNEO;NISHIDA, MASAYUKI;SUYAMA, TADAKAZU |
分类号 |
C07K14/02;C12N15/81 |
主分类号 |
C07K14/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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