| 摘要 |
The present invention provides a process for the determination of an antibody by incubation with three different reagents R1, R2 and R3, of which R1 and R3 are present in liquid phase and are bindable with the antibody, R2 is present bound to a solid phase and is bindable with R1 and R3 carries a label, separation of the solid phase from the liquid phase and measurement of the label in the solid phase, wherein as R1 there is used a conjugate of a substance specifically recognized by the antibody to be determined and a reaction component of a specific binding system, as R3 there is used a conjugate of a substance specifically recognized by the antibody to be determined and a label and as R2 there is used the other binding component of the specific binding system. The present invention also provides a composition for the determination of an antibody, wherein it contains two different soluble reagents R1 and R3 specifically bindable with the antibody, of which R1 carries a reaction component of a specific binding system and R3 carries a label and also a receptor R2 which is present in solid phase and is bindable with the receptor R1, the insoluble receptor R2 and the soluble receptor R1 being present physically separated from one another.
|
