发明名称 |
DEOXYRIBONUCLEIC ACID MOLECULES USEFUL AS PROBES FOR DETECTING DELETERIOUS GENES INCORPORATED INTO CHROMOSOMAL DNA |
摘要 |
<p>A single-stranded deoxyribonucleic acid molecule having a length of less than about 25 kb comprises at least three distinct nucleotide sequences which are the sites for incorporation into a chromosome of a deoxyribonucleic acid molecule encoding a deleterious gene. Deoxyribonucleic acid probes have been prepared from such molecules and are useful as hybridization probes for detecting chromosomal deoxyribonucleic acid which has a deoxyribonucleic acid molecule encoding a deleterious gene, i.e. oncogene, incorporated therein. A single-stranded deoxyribonucleic acid molecule derived from human chromosome 22 which is about 5.8 kb in length contains sites for incorporation of a deoxyribonucleic acid molecule encoding the oncogene c-abl derived from human chromosome 9. Deoxyribonucleic acid probes have been prepared from this molecule and used to detect the abnormal Philadelphia chromosome and chronic myelocytic leukemia.</p> |
申请公布号 |
EP0152999(B1) |
申请公布日期 |
1990.07.25 |
申请号 |
EP19850300065 |
申请日期 |
1985.01.04 |
申请人 |
US COMMERCE |
发明人 |
STEPHENSON, JOHN ROSS;GROFFEN, JOHANNES HENRIKUS;HEISTERKAMP, ELEONORA CLASSINA |
分类号 |
G01N33/50;C07H21/04;C12N15/00;C12N15/09;C12Q1/68;G01N33/566;G01N33/58 |
主分类号 |
G01N33/50 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|