METHOD OF PREPARING SUSPENSION OF HUMAN LYMPHOCYTES IN ETHANOL-ACETIC RETAINER FOR DNA EXTRACTION

摘要

FIELD: biology.SUBSTANCE: invention relates to molecular biology and can be used in biomedical research. Disclosed is a method of preparing a suspension of human lymphocytes in ethanol-acetic retainer for DNA recovery, which includes centrifugation of said suspension and removal of supernatant. Then obtained preparation is dried for 5 minutes at temperature of 55 °C, followed by adding physiological solution.EFFECT: method ensures preservation of qualitative and quantitative characteristics of sample, acceptable for further polymerase chain reaction.1 cl, 3 dwg, 1 ex