发明名称 |
Mature apoai protein production under serum free culturing conditions |
摘要 |
A method of producing a purified lipid-binding peptide which can bind to phospholipids at one or more amphipatic alpha-helical peptide regions. The method includes providing a gene coding for the peptide, and introducing the gene in expressible, heterologous form in a suitable expression system capable of synthesizing a mixture of peptides which includes the lipid-binding peptide. Addition of either endogenous or exogenous lipids to the peptide mixture forms a low-density lipopeptide complex composed of lipid and the lipid-binding peptide, and this complex can be separated easily from nonlipid-binding peptides in the peptide on the basis of its size and/or density. The method is intended particularly for scaled-up production of purified human apolipoproteins and their alpha-helical lipid-binding regions. Also disclosed are related methods for producing recombinant apolipoproteins, therapeutic lipopeptide compositions, and a stabilized lipid emulsion for nutritional therapy. Further disclosed are methods for expressing apolipoproteins or lipid-binding segments thereof in bacterial, yeast and mammalian cell expression systems, and methods for purifying lipid binding proteins, including fused recombinant proteins.
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申请公布号 |
US4943527(A) |
申请公布日期 |
1990.07.24 |
申请号 |
US19860834300 |
申请日期 |
1986.02.27 |
申请人 |
CALIFORNIA BIOTECHNOLOGY INC. |
发明人 |
PROTTER, ANDREW A.;VIGNE, JEAN-LOUIS;MALLORY, JOANNE B.;TALMADGE, KAREN D.;KANE, JOHN P. |
分类号 |
A61K31/00;C07K14/775;C12N15/62;C12N15/85;C12P21/02;C12P21/04 |
主分类号 |
A61K31/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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