RAT MONOCLONAL ANTIBODIES DIRECTED AGAINST HUMAN ANTIGENS AND PROCESSES FOR PREPARATION THEREOF

摘要

RAT MONOCLONAL ANTIBODIES DIRECTED AGAINST HUMAN ANTIGENS AND PROCESSES FOR PREPARATION THEREOF A method of producing rat monoclonal antibodies to human T-cell lymphocyte antigen epitopes other than those epitopes defined by murine monoclonal antibodies directed to the same antigen comprising (1) immunizing a rat or rat immunocompetent cells in vitro with an immunogenic amount of an antigen comprising T-cell lymphocyte markers; (2) fusing immunized cells from said rat or immunized rat immunocompetent cells with immunocytoma cells; (3) selecting hybridoma cells that produce antibody that binds to the human T-cell lymphocyte antigen epitopes other than those epitopes defined by murine monoclonal antibodies directed to the same antigen; (4) culturing the selected hybridoma cells and (5) recovering said antibody. A method of producing rat monoclonal antibodies that bind to human CD4 antigen, the method comprising: (1) immunizing a rat or rat immunocompetent cells in vitro with an immunogenic amount of an antigen comprising CD4 molecule (2) fusing immunized cells from said rat or immunized rat immunocompetent cells with immunocytoma cells; (3) selecting hybridoma cells that produce antibody that binds to human CD4 antigen; (4) culturing the selected hybridoma cells; and (5) recovering said antibody. A hybridoma that produces a rat monoclonal antibody that binds to human CD4 antigen. A rat monoclonal antibody that binds to human CD4 antigen. In a preferred embodiment the rat monoclonal antibody binds to a human CD4 antigen epitope other than those epitopes defined by murine monoclonal antibodies OKT4 and Leu3a. A rat monoclonal antibody that binds to human T-cell lymphocyte antigen epitopes other than those epitopes defined by murine monoclonal antibodies directed to the same antigen.