发明名称 |
DNA OF HUMAN PAPILLOMA VIRUS AT CERVICAL SMEAR AND DETECTION OF ITS REVELATION |
摘要 |
The invention relates to the direct detection of human papillomavirus DNA. The recently developed polymerase chain reaction has been modified in order to improve sensitivity and specificity. It is possible, by choosing suitable oligonucleotide primers (amplimers) and reaction temperatures, for individual HPV genes to be identified among the total cell 4 DNA and amplified to such an extent that non-isotopic detection is possible. In addition, the use of reverse transcription allows deliberate amplification of spliced mRNA and thus an indication of premalignant or malignant states or lesions. |
申请公布号 |
JPH02187000(A) |
申请公布日期 |
1990.07.23 |
申请号 |
JP19890293764 |
申请日期 |
1989.11.10 |
申请人 |
BEHRINGWERKE AG |
发明人 |
PEETAA SERUUTEI;JIYANETSUTO UITOKOMU;YAKOBU TSUIYURUSUTORA;ETERUUMISHIYURU DOU BIRIEERU |
分类号 |
G01N33/569;C12N15/09;C12Q1/68;C12Q1/70;C12R1/92;G01N33/574 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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