| 摘要 |
PURPOSE:To carry out the desorption and recovery of enzyme from an ion exchange material adsorbed with the enzyme, in high efficiency, without causing the deactivation of the enzyme, by using a solution of ammonium sulfate as the desorption liquid. CONSTITUTION:In the desorption of various enzymes such as acyl polyamineamide hydrolase adsorbed to an ion exchange material such as weakly acidic anion exchange material, a solution of ammonium sulfate is used as the desorption liquid. The solution is e.g. aqueous solution, buffer solution, or an aqueous solution containing an organic solvent such as acetone, ethyl alcohol, etc., and the concentration of ammonium sulfate is usually 0.025-0.5mol. |
