| 摘要 |
<p>NEW MATERIAL:A monoclonal antibody belonging to immunoglobulin G class 3, capable of specifically binding to at least sialyllacto N-fucopentaose II in the blood. USE:Reagents for use in immunoassay for sialyllacto N-fucopentaose II, pancreatic carcinoma diagnostics. PREPARATION:For example, Salmonella Minnesota bacteria incorporated with sialyllacto N-fucopentaose II is suspended into a phosphoric acid-buffered physiological saline solution, and the resulting suspension is injected into the abdomen of a BALB/C mouse to effect immunization. Three days after the final immunization, antibody-produced cells are collected from the spleen of the mouse, and fused with mouse myeloma cells followed by making a culture in a HAT medium into a hybridoma, which is then screened and put to cloning by e.g. critical dilution method followed by making a culture in the mouse abdomen and the resultant antibody is then separated from the ascites fluid, thus obtaining the objective monoclonal antibody.</p> |
