发明名称 |
SEPARATION OF LYMPHOCYTES AND MONOCYTES FROM BLOOD SAMPLES |
摘要 |
<p>This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1.060-1.065 g/cm3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.</p> |
申请公布号 |
EP0190488(B1) |
申请公布日期 |
1990.07.04 |
申请号 |
EP19850308032 |
申请日期 |
1985.11.05 |
申请人 |
BECTON, DICKINSON & COMPANY |
发明人 |
CARROLL, RICHARD JOHN;SMITH, WARD CURTIS;LUDERER, ALBERT AUGUST;ZINE, ANTHONY RALPH |
分类号 |
G01N33/50;G01N33/48;G01N33/49 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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