发明名称 |
Process and monoclonal antibody for the specific determination of pancreas alpha-amylase in the presence of saliva alpha-amylase |
摘要 |
In body fluids containing saliva alpha amylase and pancreatic alpha amylase, pancreatic alpha amylase is determined with a monoclonal antibody which specifically binds but does not inhibit saliva alpha amylase and which has a cross-reactivity of 5% or less toward pancreatic alpha amylase. The monoclonal antibody binds salvia alpha amylase to form a complex which is separated to permit determining pancreatic alpha amylase with an amylase detection system. The complex may be separated by precipitating with a precipitating agent such as an anti-antibody or protein A, or by immobilizing the monoclonal antibody on a solid carrier. The monoclonal antibody is preferably produced by immunizing a host animal with an immunogen containing aluminum hydroxide, Bordatella pertussis and native saliva alpha amylase at least 7 times over a period of at least 9 months, fusing B lymphocytes of the immunized host animal with a transforming agent to produce hybridomas, cloning and culturing the hybridomas, and screening antibodies formed.
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申请公布号 |
US4939082(A) |
申请公布日期 |
1990.07.03 |
申请号 |
US19890326885 |
申请日期 |
1989.03.21 |
申请人 |
BOEHRINGER MANNHEIM GMBH |
发明人 |
NAUJOKS, KURT W.;GERHARDT, WILLIE;HUEBNER-PARAJSZ, CHRISTA;WULFF, KARL;JUNGFER, HERBERT;LENZ, HELMUT;ALBERT, WINFRIED;WAHLEFELD, AUGUST W. |
分类号 |
G01N33/537;C12N5/10;C12N15/02;C12P21/08;C12Q1/40;C12R1/91;G01N33/573;G01N33/577 |
主分类号 |
G01N33/537 |
代理机构 |
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