| 摘要 |
<p>PURPOSE:To accomplish sure regeneration of the title plant in a short time in high efficiency by a method wherein callus is induced from the tissue pieces of a Eustoma plant, and the callus is then put to culture to differentiate indefinite buds followed by culture of the buds to form seedlings. CONSTITUTION:Seeds of a Eustoma plant (e.g. Eustoma grandiflorum) are sterilized and put to culture in a solid medium into seed seedlings. The tissue pieces prepared by finely chopping the stem/leaf parts of said seedlings are put to culture in a solid medium to induce callus. Thence, the callus is transplanted to a second solid medium to make a culture to effect differentiation of indefinite buds, which are then taken out using e.g. a forceps and transplanted to a third medium to make a culture individually to effect root generation, thus regenerating the objective seedlings. Furthermore, the seedlings can be proliferated by assimilation.</p> |
