发明名称 |
Polycationic buffers and method for gel electrophoresis of nucleic acids |
摘要 |
The present invention provides for polycationic buffers for the gel electrophoresis of and methods for obtaining improved separation and resolution of nucleic acids. In particular, the invention provides for a buffer consisting essentially of histidine for the gel electrophoresis of oligonucleotides ranging from a few to about 70 nucleotides in length. Polycationic buffers may be used for both analytical and preparative purposes and result in a nearly two-fold improvement in both separation and resolution of the nucleic acid bands. Furthermore, the use of polycationic buffers reduces the electrophoresis time by a factor of about three due to the low conductivity of the buffers.
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申请公布号 |
US4936963(A) |
申请公布日期 |
1990.06.26 |
申请号 |
US19890356590 |
申请日期 |
1989.05.23 |
申请人 |
ABBOTT LABORATORIES |
发明人 |
MANDECKI, WLODEK;HAYDEN, MARK A. |
分类号 |
G01N27/447 |
主分类号 |
G01N27/447 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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