发明名称 |
Method of effecting immunoassay by using affinity chromatography |
摘要 |
A method of effecting an immunoassay using affinity chromatography which a sample is injected into a reusable column charged with antibody applied on a solid support, an eluent is injected into the column to elute antigen bound with the antibody on the solid support, and an amount of eluted antigen is measured. In order to mitigate the influence of carry-overs of successive samples, blank values are corrected such that they include the carry-overs of previously measured samples, while elution time periods are kept constant. In one embodiment, the elution time periods are corrected such that blank values for respective samples are made constant.
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申请公布号 |
US4937200(A) |
申请公布日期 |
1990.06.26 |
申请号 |
US19870032501 |
申请日期 |
1987.03.31 |
申请人 |
OLYMPUS OPTICAL CO., LTD. |
发明人 |
KUMAZAWA, TOSHIAKI;NAKAMURA, MAKOTO;KOGURE, ERIKO |
分类号 |
G01N33/50;G01N33/543 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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