GLUCURONIDPERMEASEKODENDE GEN OG DETS ANVENDELSE SAMT REKOMBINANT-DNA-MOLEKYLE OG VAERTSCELLETRANSFORMANT INDEHOLDENDE DET

摘要

The present invention relates to the gene encoding the transport protein, glucuronide permease. Recombinant vectors encoding glucuronide permease can be used to transfect host cells. Expression of glucuronide permease by the transformants allow cellular uptake of beta -glucuronides. This system permits the detection of beta -glucuronidase activity in vivo, and can conveniently be used together with GUS gene fusions. In addition to providing an important complement to the GUS system, glucuronide permease also can be used in a method to selectively alter the permeability of cells. Because of the variety of substances which can be conjugated to beta -glucuronides, glucuronide permease provides a cellular entrance for a multitude of compounds.