Improved cryo-slammer.

摘要

A method and apparatus for ultrarapid cooling of tissue samples against a chilled cryogenic surface. The cryogenic surface (20) is enclosed in a high vacuum chamber (21) during cooling of the cryogenic surface. Dry non-condensable room temperature gas is introduced from an external source (50) to raise the chamber pressure just prior to slamming or plunging a sample against the cryogenic surface. The cryogenic surface is heated for regeneration or cleaing purposes between each successive sample.