| 摘要 |
PURPOSE:To relieve the influence of temp. and to improve the measurement accuracy of the substrate concn. in a living body forming electrode systems and an enzyme reaction layer consisting of an oxidation reduction enzyme, hydrophilic high polymer and electron acceptor on an insulating substrate and further, adding a heater and temp. control function thereto. CONSTITUTION:Conductive carbon paste is printed on an insulating substrate 1 and is dried by heating to form the electrode system consisting of a counter electrode 2 and a measuring electrode 3. An aq. soln. of carboxymethylcellulose CMC which is one kind of the hydrophilic high polymers of a cellulose system is applied on the substrate to cover the surfaces of the electrode systems 2b and 3b and is dried to form the CMC layer. A soln. prepd. by dissolving glucose oxidase as the oxidation reduction enzyme into a phosphoric acid buffer soln. of, for example, pH 5.6 is applied on the resulted CMC layer and is dried. Further, a mixture formed by mixing the fine crystals of potassium ferricyanide which is the electron acceptor and lecithin of a surfactant with toluene as an org. solvent is dropped thereon to form the potassium ferricyanide layer. The enzyme reaction layer contg. the oxidation reduction enzyme, etc., on the surface of the electrode system is formed in such a manner. |
