SACCHAROMYCES CEREVICIAE POP1

摘要

NEW MATERIAL:Saccharomyces cereviciae POP1 (FERM 10417) which is a mutant of Saccharomyces cerevisiae YNN27 and excretes protein in a high degree. USE:It is useful as a host which can excrete the protein in a high degree, when different kinds of proteins are produced through a gene recombination technique. PREPARATION:At first, PGK gene promoter, secretion signal of alpha-factor of yeast (MF alpha1) in a yeast, mouse alpha-amylase gene and the terminator area of MF alpha1 gene are inserted into a know plasmid Ylp5 to prepare plasmid pSAKO 31. The straight chain DNA of the plasmid is inserted into Saccharomyces cerevisiae YNN27 chromosome and treated with ethyl methanesulfonate to induce mutation.