| 摘要 |
The invention discloses methods for producing mature protein products. A host cell which has been co-transformed with two recombinant DNA molecules, the first of said recombinant DNA molecules comprises a fusion protein nucleotide sequence, wherein the fusion protein encoded by said coding sequence comprises a first portion, a protein product portion and a protease cleavage site portion therebetween, and the second of said recombinant DNA molecules comprising a coding sequence for a protease or an active fragment or derivative thereof, capable of cleaving amino acid encoded by the protease cleavage site portion is cultured, whereby upon culturing the cell, both the fusion protein and the protease are expressed. The protease cleaves the fusion protein at the protease cleavage site to thereby produce the protein product. In preferred embodiments of the invention, the fusion protein nucleotide sequence and the protease nucleotide sequence are incorporated into two expression vectors that are then inserted into the same cell for expression and production of the mature protein product. |
