| 摘要 |
A method of detecting an infection caused by a specific type of virus in a biological specimen, is described. The specimen is treated in a per se known manner for the purification of viral RNA and transcription of a selected RNA sequence to the corresponding cDNA, followed by a first amplification of said cDNA by the polymerase chain reaction (PCR) using a first pair of primers, optionally dilution of the PCR product and optionally a second amplification of said cDNA by PCR, then said type of virus is identified by hybridizing said amplified cDNA with a labeled probe specific for said type of virus and possibly formed hybrides are detected with the aid of the label used. The specific features are that the specific type of virus is bovine viral diarrhea virus (BVDV) and that the first pair of primers, the optional second pair of primers and the probe are all selected from synthetic oligonucleotide sequences corresponding to oligonucleotide sequences located within the gp48 nucleotide region of 1362 to 1880 of the BVDV genome. Further, new primers, new probes and a test kit for the detection of an infection caused by BVDV are disclosed. <IMAGE> |
