| 摘要 |
NEW MATERIAL:A bioactive substance MP-A produced by a Staphylococcus aureus and having the following properties; Molecular weight: 1X10<4> dalton(SDS- PAGE method), Bioactivity: significantly high activity of lymphocyte transformation to human peripheral blood mononuclear cyte, production of interleukin-2, cytotoxicity and induction of IgG production, Solubility: soluble at >=pH4 and precipitate at <=pH3.5, Adsorptivity: adsorbed on strong ion exchanger. USE:A remedy of severe infectious diseases, virus infectious diseases, malignant tumor, etc. PREPARATION:For example, Staphylococcus aureus Cowan III type is cultured and the bacterium is crushed using an ultrasonic disintegrator followed by centrifugation for separation of the supernatant. The separated supernatant is ultrafiltrated for concentration and the resultant concentrated solution is dialyzed and subsequently subjected to the affinity chromatography method using a human IgG-bonded agarose column to collect the non-adsorbed fraction. To the obtained non-adsorbed fraction ammonium sulfate is added and the resultant precipitate is collected and redissolved. The obtained solution is fractionated using a strong ion exchange column for purification, thus obtaining the objective bioactive substance MP-A. |
