TARGET NUCLEIC ACID AMPLIFICATION/DETECTION SYSTEMS

摘要

TARGET NUCLEIC ACID AMPLIFICATION/DETECTION SYSTEMS This invention relates to the use of functional reporter molecules in the detection and measurement of RNA sequences in a sample, as a determination, for example, of pathogenic disease existence or potential. The invention is predicated on the utilization of nucleotide sequences, one having a probe sequence linked to the complement of a sequence capable of initiating replication. The other is capable of hybridizing to a strand separated from the extension product of the first nucleotide sequence after hybridization to a specific target sequence. The extension product of the second hybridized nucleotide sequence serves as a template source for replication. The replication products are detected as a means for detection of target sequence.